Journal Covers


LOC-CTC (2012)

IEEE-1 (2012)

Analytical Methods-2014


Peer-reviewed Publications: 

For updated list of publication, please check Google Scholar Page

54.  Md Kabir, H Zilouchian, M Sher, and W Asghar, Development of a flow-free automated colorimetric detection assayintegrated with smartphone for Zika NS1, Diagnostics, (2020)



53.  A Makler, and W Asghar, Exosomal biomarkers for cancer diagnosis and patient monitoring, Expert Review of Molecular Diagnostics, (2020)




52.  Md Kabir, H Zilouchian, M Caputi, andW Asghar, “Advances in HIV diagnosis and monitoring, Critical Reviews in Biotechnology, (2020)






51. M Sher, W Asghar, Development of a multiplex fully automated assay for rapid quantification of CD4+ T cells from whole blood, Biosensors and Bioelectronics, vol 142, (2019)



50. S Ilyas, A Simonson, and W. Asghar,Emerging Point-of-Care Technologies for Sickle Cell Disease Diagnostics, Clinica Chimica Acta, vol 501, Pages 85-91,(2019)



49. C Coarsey, B Coleman, MA Kabir, M Sher, W Asghar, Development of a flow-free magnetic actuation platform for an automated microfluidic ELISA, RSC Advances 9 (15), 8159-8168 (2019)




48. W Asghar, M Sher, NS. Khan, JM Vyas, and UDemirci,Microfluidic Chip for Detection of Fungal Infections, ACS Omega, vol 4, issue 4, Pages 7474-7481, (2019)




47. B Coleman, C Coarsey, W Asghar, Cell Phone based Colorimetric Analysis for Point-of-Care Settings Analyst, 144, 1935-1947 (2019)




46. O Adenmosun, JK Diaka, and W Asghar, Sperm sexing with the ideal H-Y antigen, Fertility and Sterility,Consider This. (2019)


45. T Famuyiwa, J Jebelli, Z Bowers, A Bentley,D Caraballo, P Subtil, K Diaka, W Asghar, A new approach for preparing SC-514 loaded PLGA particles by single emulsion method, Journal of Medical Pharmaceutical and Allied Sciences, 8 (6), 2367-2380 (2019).



44. B Coleman, C Coarsey, Md Kabir, and W. Asghar“Point-of-care Colorimetric Analysis through Smartphone Video”, Sensors and Actuators B: Chemical, vol 282, Pages 225-231, (2019)




43.TO Famuyiwa, J Jebelli, JK Diaka, and W. Asghar“Interaction between 3-Bromopyruvate and SC-514 in prostate cancer treatment”, Journal of Cancer Prevention & Current Research, 9 (6), (2018)


42. K Rappa, J Samargia, M Sher, JS Pino, HF Rodriguez, and W. Asghar“Quantitative analysis of sperm rheotaxis using a microfluidic device”, Microfluidics and Nanofluidics 22 (9), 100, (2018)



41. C. Herrada, Md A. Kabir, R. Altamirano, and W. Asghar“Advances in Diagnostic Methods for Zika Virus Infection”, Journal of Medical Devices, (2018)



40. S. Sharma, R. Zhuang, M. Long, M. Pavlovic, Y. Kang, A. Ilyas, and W. Asghar“Circulating tumor cell isolation, culture, and downstream molecular analysis”, Biotechnology Advances, (2018)



39. S. Yu, M. Rubin, S. Geevarughese, J. S. Pino, H. F. Rodriguez and W. Asghar“Emerging technologies for home-based semen analysis”, Andrology, Vol 6, (2018)



38. M.K. Kanakasabapathy, H.J. Pandya, M.S. Draz, M.K. Chug, M. Sadasivam, S. Kumar, B. Etemad, V. Yogesh, M. Safavieh, W. Asghar, J.Z. Li, A.M. Tsibris, D.R. Kuritzkes and H. Shafiee, “Rapid, label-free CD4 testing using a smartphone compatible device”, Lab on a Chip, Issue 17, (2017)



37. C.T. Coarsey, N. Esiobu, R. Narayanan, M. Pavlovic, H. Shafiee, and W. Asghar, “Strategies in Ebola virus disease (EVD) diagnostics at the point of care”, Critical Reviews in Microbiology, (2017)







36. M. Fennell and W. Asghar“Image Sensor Road Map and Solid-State Imaging Devices”NanoWorld, Issue 4 (2017)


35. M. Sher, R. Zhuang, U. Demirci, and W. Asghar, Paper-based analytical devices for clinical diagnosis: recent advances in the fabrication techniques and sensing mechanisms”, Expert Review of Molecular Diagnostics, (2017)




34. O. Adenmosun, W. Asghar, and J Kumi-Diaka, “Sickle Cell Sperm Selection with Hb-S Mab: A Future Application for Intracytoplasmic Genotypically Selected Sperm Injection (IGSI)”, Archives of Clinical Microbiology, vol 8:1 (2017)


33. W. Asghar, M. Yuksekkaya, H. Shafiee, M. Zhang, M. O. Ozen, F. Inci, M. Kocakulak, and Utkan Demirci, Engineering long shelf life multi-layer biologically active surfaces on microfluidic devices for point of care applications, Nature Scientific Reports, vol 6, article 21163 (2016)






32. M. Safavieh, C. Coarsey, N. Esiobu, A. Memic, JM. Vyas, H. Shafiee#, and W. Asghar#, “Advances in Candida detection platforms for clinical and point-of-care applications”, Critical Reviews in Biotechnology, (# Corresponding Authors(2016)



31. W. Asghar, H. Shafiee, V. Velasco, V. R. Sah, S. Guo, R. El Assal, F. Inci, A. Rajagopalan, M. Jahangir, R. M. Anchan, G. L. Mutter, M. Ozkan, C. S. Ozkan, and U. Demirci, “Toxicology Study of Single-walled Carbon Nanotubes and Reduced Graphene Oxide in Human Sperm”, Nature Scientific Reports, vol 6, article 30270 (2016)



30. K. Rappa,  H.F. Rodriguez, G.C. Hakkarainen, R.M. Anchan, G.L. Mutter, W. Asghar, “Sperm processing for advanced reproductive technologies: Where are we today?”, Biotechnology Advances, Accepted (2016)





29. M. Safavieh, M.K. Kanakasabapathy, F. Tarlan, M. Ahmed, M. Zourob, W. Asghar#, and H. Shafiee#, “Emerging Loop-mediated Isothermal Amplification-based Microchip and Microdevice Technologies for Nucleic Acid Detection”,  ACS Biomaterials Science and Engineering”, Accepted, (# Corresponding Authors) (2016)





Figure 1


28. W. Asghar*#, R. EL Assal*, H. Shafiee, S. Pitteri, R. Paulmurugan, and U. Demirci#, “Engineering cancer microenvironments for in vitro 3-D tumor models”,  Materials Today, vol. 18, no. 10, (* Equal Contribution, # Corresponding Authors) (2015)





27. H. Shafiee*, W. Asghar*, F. Inci*, M. Yuksekkaya, M. Jahangir, M. H. Zhang, N.G. Durmus, U.A. Gurkan, D. R. Kuritzkes, and U. Demirci, “Paper and flexible substrates as materials for biosensing platforms to detect multiple biotargets,” Nature Scientific Reportsvol 5, article 8719 (* Equal Contribution) (2015)








 26. W. Asghar, V. Velasco, J.L. Kingslye, M.S. Shoukat, H. Shafiee, R.M. Anchan, G.L. Mutter, E. Tuzel, and U. Demirci, “Selection of functional human sperm with higher DNA integrity and fewer reactive oxygen species,” Advanced HealthCare Materials, vol. 3, no. 10, pp. 1671–1679 (2014)






 25. W. Asghar*, R. EL Assal*, H. Shafiee, R.M. Anchan, and U. Demirci, “Preserving human cells for regenerative, reproductive, and transfusion medicine,” Biotechnology Journal, vol. 9, no. 7, (* Equal Contribution) (2014) 






 24. A. Ilyas, W. Asghar, Y.T. Kim and S. M. Iqbal, “Parallel Recognition of Cancer Cells using Addressable Array of Solid-state Micropores,” Biosensors and Bioelectronics, pp. 343–349 (2014)





 23. A. Ilyas, W. Asghar, Y.T. Kim and S.M. Iqbal, “Electrophysiological Analysis of Biopsy Samples using Elasticity as an Inherent Cell Marker for Cancer Detection,” Analytical Methods, vol. 6, no. 18, pp. 7166-7174 (2014) Selected for Cover Article






 22. M. Islam, W. Asghar, Y.-T. Kim and S. M. Iqbal, “Cell Elasticity-based Microfluidic Label-Free Isolation of Metastatic Tumor Cells,” British Journal of Medicine and Medical Research, vol. 4, no. 11, pp. 2129-2140, (2014)






 21. L. Wang*, W. Asghar*, U. Demirci, and Y. Wan, “Nanostructured Substrates for Isolation of Circulating Tumor Cells,” Nano Today, vol 8, no. 4, pp. 374-387 (* Equal Contribution) (2013)







 20. A. Ilyas, M. Islam, W. Asghar, J. U. Menon, A. S. Wadajkar, K. T. Nguyen, S. M. Iqbal, “Salt-leaching Synthesis of Porous PLGA Nanoparticles,” IEEE Transactions on Nanotechnology, vol 12, no.6 (2013) Selected for Cover Article







 19. W. Asghar, Y. Wan, A. Ilyas, Y.-t. Kim and S. M. Iqbal, ” Electrical Fingerprinting, 3D Profiling and Detection of Tumor Cells with Solid-state Micropores,” Lab on a Chip,vol 12, no. 13, pp. 2345-2352, (2012), Selected for Cover Article




 18. W. Asghar, Y.-t. Kim, A. Ilyas, J.S. Sankaran, Y. Wan, and S.M. Iqbal, “Synthesis of Nano-Textured Biocompatible Scaffolds from Chicken Eggshell,” Nanotechnology, vol 23, no. 47, pp. 475601 (2012)






 17. A. Hafeez*, W. Asghar*, M. M. Rafique, S. M. Iqbal and A. R. Butt, “GPU-based Real-time Detection and Analysis of Biological Molecules using Solid-State Nanopores,” Journal of Medical & Biological Engineering & Computing,vol 50, no. 6, pp. 605-615 (* Equal Contribution)(2012)





 16. W. Asghar, M. Islam, A.S. Wadajkar, Y. Wan, A. Ilyas, K.T. Nguyen and S. M. Iqbal, “PLGA Micro- and Nanoparticles Loaded into Gelatin Scaffold for Controlled Drug Release,” IEEE Transactions on Nanotechnology, vol 11, no. 3, pp. 546-553, (2012), Selected for Cover Article






 15. Y. Wan, Y. Liu, P.B. Allen, W. Asghar et al., “Capture, Isolation and Release of Cancer Cells using Aptamer-Functionalized Glass Bead Array,” Lab on a Chip,vol 12, no. 22, pp. 4693-1407, (2012) Selected for Cover Article






 14. W. Asghar, “Editorial: Microfluidics and Nanotechnology for Diagnostics and Biomedical Applications,” Micro and Nanosystems, vol 4, no. 4 (2012)








 13. J. A. Billo, J. Jones, W. Asghar, R. L. Carter and S. M. Iqbal, “Viscosity and Surface-Free Energy Effects in Thermal Shrinking of Solid-State Nanopores, Applied Physics Letters (APL), vol 100, no. 23, pp. 233107 (2012)






 12. A. Ilyas, W. Asghar, A.D. Ellington, and S.M. Iqbal, “Electrical Detection of Cancer Biomarker using Aptamers with Nanogap Break-Junctions,”Nanotechnology, vol. 23, no. 27, pp. 275502 (2012)






 11. Y. Wan, J. Tan, W. Asghar, Y.-t. Kim, Y. Liu and S. M. Iqbal, “Velocity Effect on Aptamers-based Circulating Tumor Cell Isolation in Microfluidic Devices,” Journal of Physical Chemistry B, vol 115, no. 47, (2011)



 10. W. Asghar, A. Ilyas, R. B. Timmons and S. M. Iqbal, “Fabrication of Charged Nanopore Channels using Pulsed Plasma Deposition of Methacrylic Acid,” IEEE Nanotechnology, pp. 13 – 16 (2011)







 9. W. Asghar, A. Ilyas, R. R. Deshmukh, ‎S. Sumitsawan, R. B. Timmons and S. M. Iqbal, “Pulsed Plasma Polymerization for Controlling Shrinkage and Surface Composition of Solid-state Nanopores,”Nanotechnology, vol. 22, no. 28, 285304 (8pp) (2011)





 8. A. Ilyas, W. Asghar, Ehsan A. Q. Syed and S. M. Iqbal, “From Molecular Electronics to Proteonics: Break Junctions for Biomarker Detection,” in Proc. of IEEE-NIH Life Science Systems and Applications, pp. 79-82 (2011)





 7. W. Asghar, A. Ilyas, J. Billo and S.M. Iqbal, “Shrinking of Solid-state Nanopores by Direct Thermal Heating,” Nanoscale Research Letters, vol. 6, no.1, pp. 372 (2011)






 6. S. D. Vidyala, W. Asghar and S. M. Iqbal, “Biocompatible Nanolayered Polymerization of MEMS Devices,” IEEE Engineering in Medicine and Biology Society, pp 2901 – 2904 (2011)







 5. S.D. Divya,W. Asghar and S. M. Iqbal, “ Porous Organic Nanolayers for Coating of Solid-state Devices,” Journal of Nanobiotechnologyvol. 9, no. 18 (2011)






  4. J. Billo, W. Asghar and S. M. Iqbal, “An Implementation for the Detection and Analysis of Negative Peaks in an Applied Current Signal across a Silicon Nanopore,”  SPIE, pp 8031,  80312T (2011)







  3. W. Asghar, P. P. Ramachandran, A. Adewumi, M. R. Noor and S. M. Iqbal, “Rapid Nanomanufacturing of Metallic Break Junctions Using Focused Ion Beam Scratching and Electromigration,” ASME Journal of Manufacturing Science and Engineering, Special Issue on Nanomanufacturing, vol. 132, no. 3, pp 030911-1-4 (2010)




 2. W. Asghar, P. Ramachandran and S. M. Iqbal, “Integrating Engineering and Biology for Bio-Nanotechnology Curriculum,” ASEE/IEEE Frontiers in Education, pp. T2F-1 to T2F-6 (2010)





 1. A.Ramachandran, Y. Liu, W. Asghar, and S. M. Iqbal, “Characterization of DNA-Nanopore Interaction by Molecular Dynamics,” American Journal of Biomedical Sciences, Volume 1, no. 4, pp.344-351 (2009)


Book Chapters: 







 2. W. Asghar, H. Shafiee, P. Chen, S. Tasoglu, S. Guven, U.A. Gurkan, U. Demirci,”In vitro three-dimensional cancer culture model”, in ‘Cancer targeted drug delivery: Elusive dream’ (Edited by You Han Bae, Randall Mrsny, Kinam Park), Springer, NY, USA (2013).p.xx










 1. W. Asghar, J. Billo and S.M. Iqbal, “Solid-state nanopores for selective sensing of DNA,” in ‘Nanopores: Sensing and Fundamental Biological Interactions(Edited by R. Bashir, S.M. Iqbal); ISBN: 978-1-4419-8251-3, Springer, NY, USA (2011)





 Patents / IP Disclosure: 


1. U. Demirci, W. Asghar, “System and Method for Sperm Sorting”, Pub. No.: WO/2015/077333 International Application No.: PCT/US2014/066405; (2015)

2. S.M. Iqbal, A. Ilyas, W. Asghar, and Y.T. Kim “Solid-state Micropore Biosensor for High Throughput Cell Typing, Discrimination and Disease Detection,” IP Disclosure filed, (2014).

3. S.M. Iqbal, W. Asghar, and A. Ilyas,”Novel, Rapid and Inexpensive Synthesis of Nano-textured Scaffolds from Chicken Eggshells,” IP Disclosure filed, (2011).


Refereed Conference Proceedings (Abstracts)

28. M. Kabir, M. Caputi, W. Asghar, “Zika Virus Sero-cross-reactivity With Other Flaviviruses Using Envelope Protein Antibodies”, 28th Anniversary World Congress on Biosensors, Miami, Florida, USA, 12-15 June 2018

27. B. Coleman, W. Asghar, “Quantitative Cell Phone Image Processing for Disease Diagnostics”, 6Th Annual Conference of Analytix, Miami, USA, March 26-28, 2018

26. W. Asghar, “Rheotaxis based Sperm Selection for Advanced Reproductive Technologies”, 2nd World Congress on Embryology and In Vitro Fertilization, Orlando, Florida, USA, March 30-31, 2018

25. M. Sher, W. Asghar, “Development of a paper-based biosensor for the electrical quantification of CD4+ T cells”, 28th Anniversary World Congress on Biosensors, Miami, Florida, USA, 12-15 June 2018

24. A. Ataei, A.W.C. Lau, K. Rappa, and W. Asghar, “Selection of Functional Human Sperm with Microfluidic Sorting Device”, in Proceedings of APS Meeting, Los Angeles, California, March 5–9, 2018

23. M. Sher, W. Asghar, “Low-cost bioassay developement for resource-constrained settings”, Proceedings of 255th ACS National Meeting, New Orleans, LA, March 18-22, 2018

22. M.A. Kabir, M. Sher, M. Caputi, W. Asghar, “Development Of A Diagnostic Assay For Rapid Detection And Quantification Of Zika Virus”, Zika Research Symposium, Florida Department of Health, Boca Raton, FL, October 09, 2017

21. B. Coleman, C. Coarsey, W. Asghar, “Cell Phone Imaging Algorithm to Evaluate Quantitative Colorimetric Tests”, IEEE International Conference on Biomedical and Health Informatics (BHI), Orlando, Florida, USA (Feb. 16-19 2017)

20. C. Coarsey, B. Coleman, M. Sher, W. Asghar, “Development of Point-of-Care ELISA and Automated Platform for Cell Phone-based HIV Detection”, 6th Annual Out in Science, Technology, Engineering, and Mathematics (oSTEM), Denver, Colarado, USA (2016)

19. C. Coarsey, B. Coleman, M. Sher, W. Asghar, “Development of an Automated ELISA Integrated with Cell Phone-based HIV Detection”, 3rd NANOSMAT-USA (May 2016)

18. W. Asghar, “Paper and Flexible Material-based Devices for Disease Diagnosis”, Analytica, 25th International Trade Fair for Laboratory Techonology, Analysis, Biotechnology and Analytica Conference, Messe Munich, Germary, (May 2016)

17. K. Rappa, J. Samargia, and W. Asghar, “Can we select best sperm?”, Atlas of Science, 2016

16. W. Asghar, M. Yuksekkaya, M.H. Zhang, H. Shafiee, and U.Demirci, “Flexible Microfluidics for CD4+ T-Cell Capture and Counting”, IEEE EMBS Micro and Nanotechnology in Medicine Conference, Turtle Bay Resort, Oahu, HI (December 8-12, 2014)

15. W. Asghar, V.R. Sah, H. Shafiee, V. Velasco, A. Rajagopalan, M. Jahangir, R.M. Anchan, G.L. Mutter, M. Ozkan, C. Ozkan, and U.Demirci, “Toxicology of Carbon Based Nano-materials on Human Sperm: A Concentration and Time Dependent Study”, Graphene and Related Technologies, Istanbul, Trukey (October 13-14, 2014)

14. W. Asghar, A. Hafeez, A.R. Butt, and S.M. Iqbal, “Lab on Chip Platform Integrated with Graphics Processing Units for Real-time Detection of Tumor Cells”, Biomedical Engineering Society 2012 Annual Meeting (BMES), Atlanta, Georgia, USA (October 24-27, 2012)

13. W. Asghar, A. Hafeez, C.M.A. Arafat, A.R. Butt, and S.M. Iqbal, “Real-time Detection, Clustering and Analysis of Micropore Cell Translocation Data with Commercial Graphics Processor”, US-Korea Joint Symposium of Nanotechnology Workshops (JSNT), Grapevine, Texas, USA (May 1-4, 2012)

12. A. Ilyas, M.J Bari, W. Asghar, and S.M. Iqbal, “Nano-electrode Break-junctions for Electrical Detection of Cancer Biomarkers”, US-Korea Joint Symposium of Nanotechnology Workshops (JSNT), Grapevine, Texas, USA (May 1-4, 2012)

11. C.A. Arafat, S.D. Vidyala, W. Asghar and S.M. Iqbal, “Eco-friendly Deposition of Fluorinated Nanocomposites,” at International Conference on Materials Science and its Applications, Taif, Saudi Arabia (Feb 13-15, 2012)

10. Y. Wan, W. Asghar, P. B. Allen, A. D. Ellington, Y.-t. Kim and S. M. Iqbal, “Circulating Tumor Cells in Microfluidic Devices – Parameters to Increase Isolation Efficiency,” at Cancer  Detection and Diagnostics Technologies for Global Health Conference, Bethesda, MD (August 22-23, 2011)

9. W. Asghar, A. Ilyas, R.B. Timmons and S.M. Iqbal, “Polymerization of Nanopores for Controlled Surface Charges,” American Physical Society Meeting (APS), Dallas, TX, USA (March 2011)

8. S. D. Vidyala, W. Asghar and S. M. Iqbal, “Nanotextured Organic Polymer Film for Coating MEMS Devices,” at 27th Southern Biomedical Engineering Conference (SBEC), Arlington, TX, USA (Apr 29-May 1, 2011)

7. W. Asghar, A. Ilyas, S. Sumitsawan, R. B. Timmons and S. M. Iqbal, “Controlled Shrinking of Nanopores with in situ Surface Functionalization,” at 27th Southern Biomedical Engineering Conference (SBEC), Arlington, TX, USA (Apr 29-May 1, 2011)

6. J. Billo, W. Asghar and S. M. Iqbal, “Heat Treatment to Shrink Solid-State Nanopores,”American Physical Society Meeting (APS), Dallas, TX, USA (March 2011)

5. R. A. Khan, W. Asghar, A. Arafat, Y. Wan, S.M. Iqbal, “Classification of Cells using Solid-state micropores,” ACES symposium,TX, USA (March 23-24, 2011)

4. R. A. Khan, W. Asghar, Y. Wan, and S. M. Iqbal,  “Single Pore System for Classification of Cells,” at Biomedical Engineering Society 2010 Annual Meeting (BMES), Austin, TX, USA (October 6-9, 2010)

3. W. Asghar, A. Ilyas, P. Ramchandran, M.R. Noor and S.M. Iqbal, “Nano-manufacturing of Gold Break Junctions Fabricated with Focused Ion Beam Scratching and Electro-migration,” ACES symposium, TX, USA (March 25, 2010)

2. W. Asghar, S.M. Iqbal, “Biomarker Detection Using Solid State Nanopores”, UT MetroPlex Day, Arlington, TX, USA (March 6, 2009)

1. W. Asghar, S.M. Iqbal, “ Solid State Nanopores for Single Molecule Sensing & DNA Analysis,”  ACES Workshop, Arlington, TX, USA ( March 26, 2009)